phosphate buffered saline dpbs solution (Thermo Fisher)

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Thermo Fisher phosphate buffered saline dpbs solution
Phosphate Buffered Saline Dpbs Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphate+buffer+solution+dpbs/Phosphate-buffered+saline+(DPBS%2C+1X)%2C+Dulbecco's+formula/pm42174303-51-22-26
Average 99 stars, based on 1 article reviews

phosphate buffered saline dpbs solution - by Bioz Stars, 2026-09

99/100 stars

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Activation Assay:

Article Title: Comparative effects of hemodialysis modalities on microparticle induction and neutrophil activation in a randomized cross-over study
Article Snippet: The proliferation were evaluated from treated HCAECs compared to untreat controls by MTS cell proliferation assay kit (Promega, WI, USA) according to the manufacturer’s recommendation. .. Because MPs and the dialysis procedures might activate neutrophils, neutrophil activation and NETs were measured from the collected blood sample according to previous protocols., , Briefly, neutrophils from the collected blood were immediately separated by density gradient centrifugation (Ficoll-Polymorprep centrifugation; Serumwerk, Bernburg, Germany) using 800 g centrifugation for 30 min at room temperature before red blood cell (RBC) lysis by the lysis buffer (Biolegend, CA, USA), and washed twice with deionized phosphate buffer solution (DPBS) (Gibco, MA, USA). .. Then, the isolated neutrophils were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, MA, USA) containing 10% fetal bovine serum (FBS) (Gibco, MA, USA) at 37°C with 5% CO2.

Gradient Centrifugation:

Centrifugation:

Article Title: Blood from slaughter pigs can substitute foetal bovine serum (FBS) in cultivation media and Matrigel for cell attachment: an option for cultivated meat production
Article Snippet: .. Cryoprecipitate was obtained from plasma samples stored at −20 ◦C, by thawing at 4 ◦C and centrifugation at 5,000 × g for 15 min. Cryoprecipitate, and fibrin were used for coating plates and/or wells at 37 ◦C and 5% CO2 for 15 min. After 15 min, excessive material was washed with Dulbecco’s phosphate buffer solution (DPBS) (−/−) (Gibco, USA). ..

Red Blood Cell Lysis:

Lysis:

MTT Assay:

Article Title: Hydrogel nanocomposite-based breathable non-adhesive coating for oxygen sensing film used within extracorporeal perfusion system
Article Snippet: The flow cells were 3D printed using Clear v4 Resin with a FormLabs-3B 3D printer. .. Dulbecco’s phosphate buffer solution (DPBS) (pH = 7.4) and MTT were purchased from Thermo Fisher Scientific. .. 96-well flat culture plates, with coverglass bottom appropriate for cell imaging, were purchased from Corning.

Article Title: Hydrogel nanocomposite-based breathable non-adhesive coating for oxygen sensing film used within extracorporeal perfusion system.
Article Snippet: The flow cells were 3D printed using Clear v4 Resin with a FormLabs-3B 3D printer. .. Dulbecco’s phosphate buffer solution (DPBS) (pH = 7.4) and MTT were purchased from Thermo Fisher Scientific. .. 96-well flat culture plates, with coverglass bottom appropriate for cell imaging, were purchased from Corning.

Article Title: Carotenoid cellular uptake and antioxidant activity from differently cooked semolina and whole wheat pasta
Article Snippet: The fluorescent dye, 5- (and-6)-chloromethyl-2′,7′- dichlorofluorescin diacetate (CM-H2DCFDA) was acquired from Invitrogen (Eugene, OR, USA). .. Dimethyl sulfoxide (DMSO) was purchased from Tocris Bioscience (Toronto, ON, Canada) while 3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), trypsin-EDTA (0.25%), the cell culture medium, Dulbecco’s modified eagle’s medium (DMEM), low glucose, Glutamax TM supplement, pyruvate; Dulbecco’s phosphate buffer solution (DPBS) no calcium, no magnesium; penicillin-streptomycin (10,000 U/ml), hank’s balanced salts solution (HBSS), as well as the 75 cm2 polystyrene culture flask and the 96-well flat bottom polystyrene microplate, were all acquired from Thermo Fisher Scientific (Logan, UT, USA). .. Six-well plates and permeable inserts were from Nest Biotechnology (Wuxi Nest Biotechnology Co. Ltd; Jiangsu, China), fetal bovine serum (FBS) was from Cytiva (Marlborough, MA, USA), and Caco-2 cells were acquired from American Type Culture Collection (ATCC) (Manassas, VA, USA).

Clinical Proteomics:

Cell Culture:

Modification:

Article Title: Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant.
Article Snippet: .. Dulbecco’s Modified Eagle Medium (DMEM) containing phenol red, penicillin–streptomycin, EDTA-trypsin, and Dulbecco’s phosphate buffer solution (DPBS) was purchased from Gibco BRL Life Technologies (InvitrogenTM, Paisley, UK). .. Fetal bovine serum (FBS) was purchased from HyClone, USA.

Incubation:

Article Title: Cellular Cytotoxicity and Oxidative Potential of Recurrent Molds of the Genus Aspergillus Series Versicolores
Article Snippet: .. The reaction mixture consisted of 550 μL of Dulbecco’s Phosphate Buffer Solution (DPBS) (Gibco—Thermo Fisher Scientific, Waltham, MA, USA) incubated in 2 mL microtubes at 37 °C, to which 50 μL of 0.5 mM DTT (Thermo Fisher Scientific, Waltham, MA, USA) and 50 μL of spore suspension or fungal extract were added. .. Then, 100 μL of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB) (Thermo Fisher Scientific, Waltham, MA, USA) was added after 0, 15, and 30 min of contact staining the reaction medium a yellowish color with an optical density (OD) proportional to the amount of DTT remaining.

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